Overview

The InviMag® SalivaGene DNA Kit/ IG enables fully automated, true walk-away purification of genomic DNA from SalivaGene stabilized saliva samples on the InviGenius PLUS with sample volumes of up to 1.6 ml. The SalivaGene Collector tubes can directly be placed into the sample loading racks of the InviGenius for automatic sample detection. Defined and optimized protocols provide high yields of pure nucleic acids. Automatic barcode reading enables full tracking of samples and reagents.

Benefits

• High DNA yields from saliva samples without degradation
• High quality DNA from saliva performs as well as blood-derived DNA on downstream applications
• Fully automated, walk-away purification
• Barcodes enable tracking of samples and reagents

Product Characteristics

Workflow

The samples and reagents are placed in the loading rack and are identified by the automatic barcode scanner. Sample, reagents, and disposables set-up is software-supported. The sample lysis is performed in an optimized lysis buffer directly on the InviGenius PLUS in the heat incubator for efficient sample lysis of up to 90 °C. The heated lid prevents condensation and aerosol formation.
The InviGenius uses magnetic rods to transport the DNA-binding magnetic particles through the various extraction phases: Binding-Washing-Elution. The subsequent steps after lysis, such as binding of the nucleic acid onto the InviMag beads, washing and final nucleic acid elution are automatically performed in a fast and reproducible manner. No user intervention is necessary from sample loading to extracted DNA.

The purified DNA is of high quality and can be used for subsequent downstream applications:

• PCR and real-time PCR
• genotyping, STR, and SNP analysis
• sequencing

Application

Manual

MSDS