Overview

The InviMag® Free Circulating DNA Kit/ IG enables efficient, fully automated purification of cell-free circulating DNA (cfDNA) fragments from 4 ml of plasma or serum samples on the InviGenius PLUS. The InviGenius PLUS simplifies laboratory workflows by using a walk-away robotic system for a fully automated, standardized and highly efficient purification of the free circulating DNA from a variety of clinical relevant samples.

Benefits

• Concentration of cfDNA from high sample volume (4 ml) to low elution volumes (0.1ml)

• Efficient recovery of short and fragmented cfDNA

• Fully automated extraction system - high reproducibility through standardized processing

• For In Vitro Diagnostic Use (CE-IVD)*

Product Characteristics

Workflow

The DNA isolation process is based on the interaction of nucleic acids with silica coated magnetic particles at adapted buffer conditions. The InviGenius PLUS instrument automatically performs all steps of sample and reagent distribution. The DNA purification procedure is performed without any user intervention, except the initial loading of the system, thus allowing safe handling of potentially infectious samples. Sample cross-contamination and reagent cross-over is effectively eliminated by the automated purification process. The use of unique barcodes for samples and reagents avoids unwanted transpositions.

The InviGenius PLUS instrument uses magnetic rods to transport the DNA-binding magnetic particles through the various extraction phases: lysis, binding, washing and elution. 

Due to the high purity, the eluted circulating DNA is ready-to-use in a broad panel of downstream applications such as:

• Real-time PCR
• Array technologies
• Methylation detection
• Next Generation Sequencing

*) Compliance with EU Directive 98/79/EC on in vitro medical devices. Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.

Application

Manual

MSDS