Overview

The InviMag® FFPE DNA Kit/ IG enables efficient, fully automated purification of genomic DNA fragments from FFPE slides (or more) on the InviGenius PLUS. The InviGenius PLUS simplifies laboratory workflows by using a walk-away robotic system for a fully automated, standardized and highly efficient DNA purification.

Benefits

• Fully automated standardized DNA extraction from FFPE slides

• No manual pretreatment like deparaffination or formalin removal required

• Reduction of pure hands-on time from 60 min to 10 min

• Efficient recovery of short and fragmented ready-to-use DNA

• Proprietary validated qPCR assay available for quantification of extracted DNA

Product Characteristics

Workflow

The DNA isolation process is based on the deparaffination step and the interaction of nucleic acids with silica coated paramagnetic particles in optimal buffer conditions. 

The InviGenius Plus performs automatically all lysis, washing and elution steps of reagent distribution. The DNA purification procedure is performed without any user intervention, except any sample pretreatment and the initial loading of the system. Sample cross-contamination and reagent cross-over is effectively eliminated. The usage of unique bar codes for samples and reagents avoids unwanted transpositions.
The InviGenius Plus uses magnetic rods to transport the DNA-binding paramagnetic particles through the various extraction phases: deparaffination-lysis-bind-wash-elute. Eliminating the direct liquid handling and increasing the automation level results in a fast, reliable and robust technique.
Due to the high purity, the eluted circulating DNA is ready-to-use in a broad panel of downstream applications such as:

• Real-time PCR

• Array technologies

• Methylation detection

• Next Generation Sequencing

Application

Manual

MSDS